Relationship between Cytokines and Spinal mu Opioid Receptor Expression during Adjuvant-induced Arthritis in Rats
Elaheh Tekieh, Jalal Zaringhalam, Zeinab Akhtari
Annual Research & Review in Biology · pp. 1854–1866 · Published 22 Feb 2014
10.9734/ARRB/2014/8512Abstract
Aims: It is now clear that cytokines such as interleukins play a fundamental role in inflammatory processes. Cytokines now offer alternative targets for therapeutic intervention and may be useful as predictive biomarkers of disease. In this study, we aimed to evaluate the relationship between cytokines and spinal mu opioid receptor expression during adjuvant-induced arthritis (AA) in rats. Methodology: AA was induced by a single subcutaneous injection of CFA into the rats’ hindpaw. Anti-IL-6 and IL- 10 were administered daily during the 21 days of study. Hyperalgesia and edema were assessed on days 0, 3, 7, 14 and 21 by radiant heat and plethysmometer respectively. Spinal mu opioid receptor (MOR) expression was detected by Western blotting. Results: Daily administration of neutralizing doses of anti-IL-6 caused a significant decrease in serum IL-10 level in AA rats, while treatment with anti-IL10 antibody in the AA rats did not significantly change serum IL-6 level. Anti-IL-10 antibody administration caused a significant increase in paw edema and hyperalgesia, while anti-IL-6 treatment decreased heperalgesia on days 3 and 7 but caused a significant increased in hyperalgesia on days 14 and 21 in AA rats. Administration of neutralizing dose of anti-IL10 antibody in AA rats did not cause significant variation of spinal MOR expression in comparison to IL-6 antibody treatment which induced significant reduction in spinal MOR expression. Conclusion: Together these results suggest that some of the anti-hyperalgesic effects of serum IL-6 during the chronic phase of inflammation may be mediated by increased IL-10 levels.
References (26)
- 1 Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes. [DOI]
- 2 Interleukin-6: An Overview [DOI]
- 3 Activation and signaling of the p38 MAP kinase pathway [DOI]
- 4 The soluble interleukin 6 receptor: mechanisms of production and implications in disease [DOI]
- 5 Activation of p38 mitogen‐activated protein kinase in spinal microglia is a critical link in inflammation‐induced spinal pain processing [DOI]
- 6 The Role of Interleukin-6 in Nociception and Pain [DOI]
- 7 Inhibition of IL-1, IL-6, and TNF-α in immune-mediated inflammatory diseases [DOI]
- 8 Elevated interleukin-10 levels in patients with rheumatoid arthritis [DOI]
- 9 SIMVASTATIN ATTENUATES MICROGLIAL CELLS AND ASTROCYTE ACTIVATION AND DECREASES INTERLEUKIN-1B LEVEL AFTER TRAUMATIC BRAIN INJURY [DOI]
- 10 Leptomeningeal cells activate microglia and astrocytes to induce IL-10 production by releasing pro-inflammatory cytokines during systemic inflammation [DOI]
- 11 Interleukin 10 treatment for rheumatoid arthritis [DOI]
- 12 Differential Activation of p38 and Extracellular Signal-Regulated Kinase in Spinal Cord in a Model of Bee Venom-Induced Inflammation and Hyperalgesia [DOI]
- 13 Cellular events during arthritis-induced hyperalgesia are mediated by Interleukin-6 and p38 MAPK and their effects on the expression of spinal mu-opioid receptors [DOI]
- 14 Interleukin-4 and interleukin-10 regulate IL1-? induced mouse primary astrocyte activation: A comparative study [DOI]
- 15 Expression of Opioid Receptors During Peripheral Inflammation [DOI]
- 16 Effects of the bisphosphonate ibandronate on hyperalgesia, substance P, and cytokine levels in a rat model of persistent inflammatory pain [DOI]
- 17 Increased serum IL-6 level time-dependently regulates hyperalgesia and spinal mu opioid receptor expression during CFA-induced arthritis.
- 18 Achillea santolina reduces serum interlukin-6 level and hyperalgesia during complete Freund's adjuvant-induced inflammation in male Wistar rats [DOI]
- 19 IL ‐1β and IL ‐6 activate inflammatory responses of astrocytes against N aegleria fowleri infection via the modulation of MAPK s and AP ‐1 [DOI]
- 20 Anti-inflammatory and anti-hyperalgesic activities of Stachys athorecalyx extracts on CFA-induced inflammation [DOI]
- 21 Expression of interleukin 10 mRNA and protein by synovial fibroblastoid cells.
- 22 Relationship between serum IL10 level and p38MAPK enzyme activity on behavioral and cellular aspects of variation of hyperalgesia during different stages of arthritis in rats [DOI]
- 23 Mac-1+ myelopoiesis induced by CFA: a clue to the paradoxical effects of IFN-γ in autoimmune disease models [DOI]
- 24 Inhibitory effects of interleukin‐10 on synovial cells of rheumatoid arthritis [DOI]
- 25 INVESTIGATION OF THE RELATION BETWEEN THE HYPOTHALAMUS-PITUITARY-ADRENALAXIS, IL-6 AND HYPERALGESIA DURING RHEUMATOID ARTHRITIS IN MALE RATS
- 26 Interleukin-6 and rheumatic diseases. [DOI]
Cited by 7
Mona Taghizadeh, N. Maghsoudi, H. Manaheji · Heliyon · 2021
S. Golabi, J. Zaringhalam, H. Manaheji · Medical Journal of The Islamic Republic of Iran · 2020
Mahdi Shadnoush, V. Nazemian, H. Manaheji · Basic and Clinical Neuroscience · 2018
Mahdi Shadnoush, V. Nazemian, H. Manaheji · 2016
S. Golabi, J. Zaringhalam, H. Manaheji · 2016
S. Khashaee, H. Manaheji, N. Nikzad · 2016
Showing 6 of 7 known citations — external sources report more than can currently be individually listed.
Related research
- Assessing Respiratory Inflammation among Children Living Near to Non-sanitary Landfill Using Interleukin-6 (IL-6) — shares topic coverage
- Role of Serum Adiponectin, IL-6 and Hs CRP in Nonalcoholic Fatty Liver Egyptian Patients — shares topic coverage
- Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanisms — shares topic coverage
- NF-κB, TNF-α and IL-6 Levels in Liver and Kidney of High-Fructose-Fed Rats — shares topic coverage
- Vitamins C and E Alleviate Nephrotoxicity-induced by Potassium Bromate in Rats — shares topic coverage
Article metrics
Real usage data collected on this platform.
56
Page views
0
PDF downloads
5
Outbound clicks
7
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.