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Research Article Open access CC BY 4.0

Role of CRISPR in Crop Improvement: A Review

Hassan Raza, Muhammad Ashar Abdullah, Samar Khaliq, Muhammad Haider Sajjad, Mehboob Elahi, Muhammad Raffay Khan, Abdullah Siddique

Asian Journal of Research in Agriculture and Forestry · pp. 12–19 · Published 22 Mar 2024

10.9734/ajraf/2024/v10i2280

Abstract

The meals and agriculture sectors have witnessed good sized advancements due to the state-of-the-art improvements in agricultural biotechnology and genetic engineering, that have more advantageous the essential characteristics of plant agronomic tendencies. A extensively used technique for inducing focused deletions, insertions, and precise sequence adjustments throughout numerous species and mobile kinds is collection-particular nucleases (SSNs)-based centered genome editing. Commercial adoption of genome modifying gear, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and siRNA-mediated RNA interference, has been significant. However, the whole landscape of lifestyles sciences underwent a paradigm shift with the invention of the CRISPR/Cas9 machine as a flexible tool for genome modifying. Initially recognized as a virological defense DNA segment in bacteria and archaea, the clustered Regularly interspaced short palindromic repeats (CRISPR) machine has revolutionized molecular biology. Through modern molecular organic strategies, CRISPR/Cas9 enables unique modifications in any crop species. Its efficacy, reproducibility, and specificity have earned CRISPR/Cas9 the moniker of a "leap forward" in biotechnology.

Genome editing CRISPR/Cas crop improvement gene function palindromic commercial adoption crop species

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Cited by 9

Recent advances of CRISPR-based genome editing for enhancing staple crops

Feng Chen, Lu Chen, Zhao Yan · Frontiers in Plant Science · 2024

Tailoring traits in agricultural and horticultural crops using CRISPR-Cas9 for enhanced abiotic, biotic, and consumer-favored attributes

Ekta Patel, Piyali Das, Somak Hazra · In Vitro Cellular & Developmental Biology - Plant · 2025

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