Simultaneous Determination of Hyoscine N- Butyl Bromide and Paracetamol by RP-TLC Spectrodensitometric Method
Nouruddin W. Ali, Mohammed Gamal, Mohammed Abdelkawy
Journal of Pharmaceutical Research International · pp. 472–484 · Published 2 May 2013
10.9734/BJPR/2013/3522Abstract
Aims: A simple RP-TLC Spectrodensitometric method was developed for determination of Hyoscine N-Butyl Bromide (HBB) and Paracetamol (PAR) either in bulk powder or in their pharmaceutical preparation. Study Design: Validation study. Methodology: In this method, HBB and PAR were separated on RP-18 W/ UV254 TLC plates using developing mobile phase consisting of methanol: citrate buffer (pH=1.5): triflouroacetic acid (70:30:0.1, by volume) at room temperature. Experimental conditions such as band size, slit width, different developing systems and scanning wavelength were carefully studied and the optimum conditions were selected. The obtained bands were then scanned at 210 nm. The two drugs were satisfactorily resolved with RF 0.60 ± 0.02 for HBB and 0.81 ± 0.02 for PAR. The validation of spectrodensitometric method was done regarding linearity, accuracy, precision, and specificity. Results: Linearity of the proposed methods was evaluated and it was found to lie within the concentration range of 2.0-12.0 µg.band-1 for HBB and 2.0-14.0 µg.band-1 for PAR. Conclusion: The proposed method was successfully applied for determination of HBB and PAR in pure form and in their different pharmaceutical formulations. The method proved to be specific, accurate and selective.
References (16)
- 1 Selective spectrophotometric determination of p-aminophenol and acetaminophen [DOI]
- 2 Determination of Paracetamol in pure form and in dosage forms using N,N-dibromo dimethylhydantoin [DOI]
- 3 Determination of losartan and its degradates in COZAAR® tablets by reversed-phase high-performance thin-layer chromatography [DOI]
- 4 High-performance liquid chromatographic determination of atropine and atropine-like alkaloids in pharmaceutical preparations with indirect conductometric detection [DOI]
- 5 DETERMINATION OF HYOSCINE N-BUTYL-BROMIDE, LIDOCAINE HYDROCHLORIDE, AND PARACETAMOL IN INJECTION FORMS USING SOLID-PHASE EXTRACTION, HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, AND UV-VIS SPECTROPHOTOMETRY [DOI]
- 6 Development of membrane electrodes for the selective determination of hyoscine butylbromide [DOI]
- 7 ANALYSIS OF SYNTHETIC GASTROINTESTINAL DRUGS IN ADULTERATED TRADITIONAL CHINESE MEDICINES BY HPCE [DOI]
- 8 Nonaqueous versus aqueous capillary electrophoresis for the dosage of N-butylscopolamine in various pharmaceutical formulations [DOI]
- 9 Reversed‐Phase Thin‐Layer Chromatography of Three New Oral Antidiabetics and Densitometric Determination of Pioglitazone [DOI]
- 10 New Sensitive Method for the Analysis of Some Non UV Absorbing Quaternised Compounds [DOI]
- 11 Development and validation of a UPLC‐ESI‐MS/MS method for the determination of N‐butylscopolamine in human plasma: Application to a bioequivalence study [DOI]
- 12 Symmetric and Asymmetric Hyoscine Membrane Sensor for Determination of Hyoscine Butyl Bromide in Pharmaceutical Formulation and Biological Fluids; A Computational Study [DOI]
- 13 Sensors membrane electrodes for sensitive determination of hyoscine butylbromide in pharmaceutical formulation and in human plasma
- 14 COMPARISON OF NP-TLC AND RP-TLC WITH DENSITOMETRY TO QUANTITATIVE ANALYSIS OF TOCOPHEROL ACETATE IN PHARMACEUTICAL PREPARATION [DOI]
- 15 REVERSED-PHASE ION-PAIR THIN LAYER CHROMATOGRAPHY OF SOME ALKALOIDS [DOI]
- 16 COMPARISON OF NP-TLC AND RP-TLC WITH DENSITOMETRY TO QUANTITATIVE ANALYSIS OF IBUPROFEN IN PHARMACEUTICAL PREPARATIONS [DOI]
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