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Research Article Open access CC BY 4.0

Stability of Insulin on Polycaprolactone Nanoparticles as a Function of Surface Properties

Nader I. Namazi

Journal of Pharmaceutical Research International · pp. 296–309 · Published 14 Dec 2021

10.9734/jpri/2021/v33i56B33956

Abstract

The purpose of this research was to formulate insulin-loaded polycaprolactone (PCL) nanoparticles and evaluate structural stability of the protein using fluorescence spectroscopy. The size and morphology of the nanoparticles were characterized using dynamic light scattering (DLS) and scanning electron microscopy (SEM). Fluorescence emission data revealed that insulin is most stable with multilayer adsorption at pH close to its isoelectric point (IEP). The obtained particle size ranged from 130-140 nm+22 (SD). The loading amount of insulin onto the PCL nanoparticles was low at pH 7.4 and relatively high at pH 5.3. The adsorption phenomenon of protein onto hydrophobic nanoparticles provides a promising noninvasive carrier system for insulin.

Protein adsorption Insulin PCL nanoparticles Fluorescence spectra dynamic light scattering

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Cited by 2

Oral insulin delivery: Barriers, strategies, and formulation approaches: A comprehensive review

S. Spoorthi Shetty, Praveen Halagali, Asha P. Johnson · International Journal of Biological Macromolecules · 2023

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