Storage Stability and Probiotic Viability of Foxtail Millet Probiotic Powder Enriched with High Protein and Fiber
Aiswarya Velekat Santhosh, Sweety, Asif Ali T S, Ramachandra B
Journal of Advances in Microbiology · pp. 27–36 · Published 14 Jan 2025
10.9734/jamb/2025/v25i1892Abstract
Aims: The study aimed to evaluate the storage stability of foxtail millet probiotic powder enriched with high protein and dietary fiber under two storage conditions: room temperature (27 ± 1°C) and refrigeration temperature (7 ± 1°C). Study Design: This was an experimental, laboratory-based study. Place and Duration of Study: The study was conducted at the Department of Dairy Microbiology, Dairy Science College, Hebbal, Bengaluru, Karnataka, India, between January 2024 and October 2024. Methodology: Foxtail millet probiotic powder enriched with high protein and dietary fiber was stored in polypropylene sachets under two storage conditions: room temperature and refrigeration temperature. Samples were systematically drawn at 7-day intervals and analyzed for key quality parameters, including probiotic viability, microbial contaminants, water activity, moisture content, alcoholic acidity, and pH. Statistical analyses were performed using Analysis of Variance (ANOVA), with a 5% significance level. A critical difference (CD) value was employed to determine the statistical significance of observed variations between treatments over the storage period. Results: The storage stability of the optimized foxtail millet probiotic dry mix was assessed at both room temperature (27 ± 1°C) and refrigeration (7 ± 1°C) until spoilage or decline in probiotic viability below the threshold value. Probiotic viability declined significantly under both conditions, with a reduction from 11.04 log₁₀ CFU/g to 8.88 log₁₀ CFU/g at room temperature and 8.81 log₁₀ CFU/g under refrigeration. Water activity and moisture content increased over time, reflecting the hygroscopic nature of the mix, while alcoholic acidity rose significantly in both storage conditions. Despite these changes, the mix maintained probiotic viability above the therapeutic threshold (9 log₁₀ CFU/g) for 84 days at room temperature and 105 days under refrigeration, demonstrating its potential as a stable functional food product. Conclusion: The storage study of foxtail millet probiotic powder demonstrated its stability under both refrigeration and ambient conditions, with probiotic viability exceeding the 1 billion CFU/g threshold for up to 105 days and 84 days, respectively. Significant changes in water activity, moisture content, alcoholic acidity, and pH were observed, highlighting the product’s dynamic response to storage conditions.These results support the feasibility of developing shelf-stable, high-protein, high-fiber probiotic millet powders for the health-conscious market.
References (8)
- 1 The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic [DOI]
- 2 Effects of storage conditions on milk powder properties [DOI]
- 3 Formulation of prebiotic, low glycemic index millet soups using foxtail, barnyard and kodo millet [DOI]
- 4 Formulation and validation of probioticated foxtail millet laddu as a source of antioxidant for biological system using response surface methodology [DOI]
- 5 Optimization of Soaking Conditions to Enhance Protein and Reduce Phytate Content in Foxtail Millet (Setaria italica) [DOI]
- 6 Formulation of Foxtail Millet Probiotic Powder Enriched with High Protein and Fibre [DOI]
- 7 Effect of Different Treatments on Chemical Components of Fermented Probiotic Millet Dried Mix [DOI]
- 8 Effect of Germination, Drying, Milling and Sieving on the Nutritional Quality of Foxtail Millet (Setaria italica) [DOI]
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