Skip to content
Research Article Open access CC BY 4.0

Structural and Rheological Properties of Exopolysaccharides Produced by Some Lactic Acid Bacterial Strains Isolated from Palm Wine

O. L. Adamu-Governor, T. A. Shittu, O. R. Afolabi, A. I. Elijah, C. N. Opara, S. V. A. Uzochukwu

Biotechnology Journal International · pp. 1–14 · Published 24 Apr 2023

10.9734/bji/2023/v27i2674

Abstract

Aims: This study evaluated the physical, chemical and rheological properties of exopolysaccharides (EPSs) produced by Lactic Acid Bacteria (LAB) isolated from palm wine. Materials and Methods: EPSs from palm wine LAB strains were produced on 6% sucrose broth, purified and freeze-dried prior to analyses.  Molecular weights (MW), rheological and structural composition (functional groups) of the EPSs were determined using standard methods and Fourier transform infrared spectroscopy (FTIR). Results: The average MW of the EPSs ranged from 2.02×106 to 6.53×106 Da while the flow index (n) values ranged from 0.03-3.13 at 0.2%, 0.06-1.51 at 0.4%, 0.38 - 1.85 at 0.6%, 0.14 - 2.26 at 0.8% and 0.55 - 6.42 at 1% concentrations at elevated temperatures for EPS solutions from the ten LAB species. The FTIR spectrum revealed prominent peaks of various groups of OH (3420 cm-1) and CH3 bending (2090 cm-1) in all the EPSs corresponding to both hydroxyl and amine groups, and aliphatic C-H bonds, respectively. EPS synthesized by Leuconostoc lactis, Lactococcus lactis subsp lactis and Lactiplantibacillus plantarum showed weak absorption peaks (1148 – 1145 cm-1) indicating the C-O-C and C-O bonds, while absorption peaks of Lactobacillus lactis, Lactobacillus acidophilus and Lactiplantibacillus plantarum (1267 – 1253 cm-1) indicated O- acetyl ester and other non-sugar components. Conclusions: The FTIR spectra, rheological properties and molecular weight of EPSs synthesized by the ten LAB strains indicated potentials that could be exploited in different industrial applications, and as stabilizers in food industries

Exopolysaccharides molecular weight lactic acid bacteria fourier transform infrared spectroscopy palm-wine viscosity

References (45)

  1. 1 Hydrocolloids as thickening and gelling agents in food: a critical review [DOI]
  2. 2 An overview of the functionality of exopolysaccharides produced by lactic acid bacteria [DOI]
  3. 3 Microbial Cellulose: Fermentative Production and Applications
  4. 4 Properties and applications of xanthan gum [DOI]
  5. 5 Exopolysaccharides from lactic acid bacteria: perspectives and challenges [DOI]
  6. 6 Rheological properties of selected hydrocolloids as a function of concentration and temperature [DOI]
  7. 7 New perspectives for Lactobacilli exopolysaccharides [DOI]
  8. 8 Invited Review: Methods for the Screening, Isolation, and Characterization of Exopolysaccharides Produced by Lactic Acid Bacteria [DOI]
  9. 9 Physical characterization of exopolysaccharide produced by Lactobacillus plantarum KF5 isolated from Tibet Kefir [DOI]
  10. 10 Heteropolysaccharides from lactic acid bacteria [DOI]
  11. 11 Isolation and characterization of extracellular polymeric substances from micro-algae Dunaliella salina under salt stress [DOI]
  12. 12 Purification and characterization of an extracellular polysaccharide from haloalkalophilic Bacillus sp. I-450 [DOI]
  13. 13 Use of FT-IR spectroscopy as a tool for the analysis of polysaccharide food additives [DOI]
  14. 14 Structure-function relationships in microbial exopolysaccharides [DOI]
  15. 15 Use of ATR/FTIR spectrometry to study fouling of microfiltration membranes by natural waters [DOI]
  16. 16 Production, characterization and In vitro antioxidant activities of exopolysaccharide from Weissella cibaria GA44 [DOI]
  17. 17 Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium [DOI]
  18. 18 Rheological and emulsifying properties of an exopolysaccharide produced by potential probiotic Leuconostoc citreum-BMS strain [DOI]
  19. 19 EPS-Producing Lactobacillus plantarum MC5 as a Compound Starter Improves Rheology, Texture, and Antioxidant Activity of Yogurt during Storage [DOI]
  20. 20 Identification of some bacterial cell components by FT-IR spectroscopy [DOI]
  21. 21 Production of a Novel Extracellular Polysaccharide by Lactobacillus sake 0-1 and Characterization of the Polysaccharide [DOI]
  22. 22 Influence of the pyruvate content of xanthan on macromolecular association in solution [DOI]
  23. 23 Role of exopolysaccharides produced by Lactococcus lactis subsp. cremoris on the viscosity of fermented milks [DOI]
  24. 24 Quantification and Characterization of Exopolysaccharides from Bacillus subtilis (MTCC 121)
  25. 25 Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization [DOI]
  26. 26 Optimization of exopolysaccharide yields in sourdoughs fermented by lactobacilli [DOI]
  27. 27 Probiotic properties of bifidobacteria and lactobacilli isolated from local dairy products [DOI]
  28. 28 Isolation, screening and optimization of exopolysaccharide producing bacterium from saline soil
  29. 29 Physicochemical analyses of the exopolysaccharides produced by a marine biofouling bacterium, Vibrio alginolyticus [DOI]
  30. 30 Exopolysaccharides from lactic acid bacteria: structure, production and technological applications.
  31. 31 Investigation of water transfer across thin layer biopolymer films by infrared spectroscopy [DOI]
  32. 32 Influence of hydration of food additive polysaccharides on FT-IR spectra distinction [DOI]
  33. 33 Preliminary characterization of exopolysaccharides produced by a marine biofilm-forming bacterium Pseudoalteromonas ruthenica (SBT 033) [DOI]
  34. 34 Exocellular polysaccharides produced by lactic acid bacteria [DOI]
  35. 35 Structural analysis by 13C- nuclear magnetic resonance spectroscopy of glucan extracted from natural palm wine [DOI]
  36. 36 Highly efficient synthesis of exopolysaccharides by Lactobacillus curvatus DPPMA10 during growth in hydrolyzed wheat flour agar [DOI]
  37. 37 Experimental Investigation of Crude Oil–Xanthan Emulsions Flow Behavior [DOI]
  38. 38 Characterization of Two Extracellular Polysaccharides from Marine Bacteria [DOI]
  39. 39 Some chemical and physical properties of extracellular polysaccharides produced by Butyrivibrio fibrisolvens strains [DOI]
  40. 40 Comparative Study of Commercial Starter and Gum-producing Lactic Acid Bacteria Isolated from Palm-sap on Yoghurt [DOI]
  41. 41 Effect of Manufacturing Conditions on Rheology of Banana Gelified Milk: Optimization of the Technology [DOI]
  42. 42 Hydration of polysaccharide hyaluronan observed by IR spectrometry. I: Preliminary experiments and band assignments
  43. 43 Physical Properties of Solutions of Polysaccharides [DOI]
  44. 44 Modeling for Exopolysaccharide Production by Alcaligenes faecalis B14
  45. 45 COMPARATIVE IDENTIFICATION APPROACH FOR GUM-PRODUCING LACTOBACILLI IN PALM WINE [DOI]

Cited by 4

Emerging Insights into Wine Polysaccharides: Mechanisms, Evolutions and Applications in Wine Flavor Management

Zhen-Yu Yang, Linna Song, Yue-Tong Hao · Trends in Food Science & Technology · 2026

Showing 2 of 4 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

55

Page views

0

PDF downloads

7

Outbound clicks

4

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.