Structural Elucidation of Some Antibiotics against Wound Bacterial Pathogens from Penicillium Species Using Agrowastes
Ikechukwu Harmony Iheukwumere, Chidiogo Marigold Iheukwumere, Obianuju Helen Obiefuna, Bright Chukwuebuka Unaeze, Michael Ikechukwu Nwike, Amaka Olivia Obianom, Nnenna Nelly Onyemekara, Chimamkpam Henry Nnadozie, Edwin Uchechukwu Igboanugo, Victor Echezona Ike, Nnamdi Enoch Nwakoby
Journal of Pharmaceutical Research International · pp. 102–114 · Published 20 Mar 2025
10.9734/jpri/2025/v37i37673Abstract
Aim: This study was undertaken to carry out structural elucidation of some antibiotics from Penicillium species using Agro-wastes. Methodology: Penicillium species were isolated from garden soil samples, and grown in formulated mycological medium that comprises Phoenix dactylifera (PD) fruits, Chrysophyllum albidum (CA) fruits, Glycine max (GM) peel and Musa paradisiaca (MP) peel, considering varying conditions (pH, temperature, carbon and nitrogen sources). The extracted antibiotics were exposed to wound pathogens using agr welled diffusion method. The industry fractions were precipitated, eluted, purified and elucidated using column, thin layer and gas chromatographic coupled with mass spectrophotometer (GC-MS) techniques. Results: The fungi isolated were Penicillium digitatum strain PdWO3 (PdP3) and Penicillium Oxalicum strain LIR1 (POL1). The fungal isolates showed significant (P<0.05) growth and production of antibiotics at pH 7.0, temperature (25), when the carbon and nitrogen sources were sugar from PD and NOdz respectively. The fractions; oleic acid (P1) > hexacosanoic acid (P2) > hexadecenoic acid (P3) > 10-octaecenoic acid (P4) > 9-octadecenoic acid (Z) methyl esters (P5) > 9-octadecenoic acid (Z)-2hydroxy-1-(hydroxymethyl) ethyl ester, eluted and purified from PdP3 showed significant (P<0.05) inhibitory activities against wound bacterial pathogens; Pseudomonas aeruginosa PGN5 (PAP5) and Streptococcus pyogenes A1 (SPA1). Similarly, the fractions; oleic acid (L1) > hexacosanoic acid (L2) > E-11-tridece-1-ol-acetate (L4) > 1-octadecene (L3) > 2H-A zepin-2-one hexa-hydro-1-methyl (L5) eluted from POL1 exhibited significant (P<0.05) inhibitory activities against wound bacterial pathogens. Conclusion: Therefore, this study has proven that the fractions eluted from PdP3 and POL1 showed pronounced activities against wound bacterial pathogens, and could be used as newer antibiotics against wound infections.
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