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Research Article Open access CC BY 4.0

Study of the Gut Enterotypes in Egyptian Children with Autism Spectrum Disorder

Shwikar M. Abdelsalam Ahmed, Azza Mahmoud El-Hefnawy, Hanan Azouz, Marwa Ahmed Meheissen, Mona Hamdy, Yara Safwat Roshdy, Ahmed Elsayed Ibrahim

Microbiology Research Journal International · pp. 1–9 · Published 17 May 2018

10.9734/MRJI/2018/41460

Abstract

Background: Gut microbiota distribute into three enterotypes named the Bacteroides, Prevotella and Ruminococcus. While each person’s microbial “fingerprint” is unique, there are specific patterns seen in those that are healthy and those that have specific illnesses. Aims: The aim of the present study is to identify the enterotypes that are likely related to ASD as well as their possible role in the severity of the disease and gastrointestinal symptoms Subjects & Methods: The study included 41 ASD patients, 45 of their neurotypical siblings and 45 unrelated controls. Autism severity was assessed using Childhood autism rating scale (CARS). Gastrointestinal symptoms were assessed by a modified six-item Gastrointestinal Severity Index (6-GSI) questionnaire. Quantitative real-time SYBR green PCR was done for the identification and quantitation of the dominant enterotypes. Results: Enterotype 1 (Bacteriodes) was the most prevalent enterotype accounting for 76.7%, 75.6% and 57.8% in patients with autism, their neurotypical siblings and unrelated controls respectively. Enterotype 3 (Ruminococcus dominant), it was detected nearly equal in the three groups. Enterotype 2 (Prevotella dominant), it was the least enterotype encountered in ASD group (4.9%) compared to 8.9% and 22.2% in the siblings and the unrelated control respectively. About 75.6% of ASD patients shared the same enterotype with their siblings. There was no significant difference between the three enterotypes as regards the CARS or 6-GSI. Conclusion: There was no significant difference in the distribution of enterotypes in all study groups. Therefore, collapsing the whole microbiome variations into dominant enterotypes was not appropriate to identify disease association or to be used as a disease biomarker. Further studies of individual bacterial species may be more accurate to determine if there are any possible correlations between the gut microbiome and the gastrointestinal dysfunction in ASD patients.  

Autism gut microbiome gut enterotypes SYBR green real-time PCR.

References (26)

  1. 1 Enterotypes of the human gut microbiome [DOI]
  2. 2 Linking Long-Term Dietary Patterns with Gut Microbial Enterotypes [DOI]
  3. 3 Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa [DOI]
  4. 4 Toward objective classification of childhood autism: Childhood Autism Rating Scale (CARS) [DOI]
  5. 5 Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set [DOI]
  6. 6 Gastrointestinal flora and gastrointestinal status in children with autism – comparisons to typical children and correlation with autism severity [DOI]
  7. 7 Reduced Incidence of Prevotella and Other Fermenters in Intestinal Microflora of Autistic Children [DOI]
  8. 8 Pyrosequencing study of fecal microflora of autistic and control children [DOI]
  9. 9 Evaluation, Diagnosis, and Treatment of Gastrointestinal Disorders in Individuals With ASDs: A Consensus Report [DOI]
  10. 10 Dietary quality among men and women in 187 countries in 1990 and 2010: a systematic assessment [DOI]
  11. 11 Effect of inulin on the human gut microbiota: stimulation of Bifidobacterium adolescentis and Faecalibacterium prausnitzii [DOI]
  12. 12 Gastrointestinal Problems in Children with Autism, Developmental Delays or Typical Development [DOI]
  13. 13 Altered gut microbiota and activity in a murine model of autism spectrum disorders [DOI]
  14. 14 Childhood Autism Rating Scale (CARS) and Autism Behavior Checklist (ABC) Correspondence and Conflicts with DSM-IV Criteria in Diagnosis of Autism [DOI]
  15. 15 Autism and gastrointestinal symptoms [DOI]
  16. 16 Relation of childhood gastrointestinal disorders to autism: nested case-control study using data from the UK General Practice Research Database [DOI]
  17. 17 Comparison of Fecal Microbiota in Children with Autism Spectrum Disorders and Neurotypical Siblings in the Simons Simplex Collection [DOI]
  18. 18 An investigation of comorbid psychological disorders, sleep problems, gastrointestinal symptoms and epilepsy in children and adolescents with Autism Spectrum Disorder [DOI]
  19. 19 Gut bacteria in children with autism spectrum disorders: challenges and promise of studying how a complex community influences a complex disease [DOI]
  20. 20 Familial Recurrence of Autism Spectrum Disorder: Evaluating Genetic and Environmental Contributions [DOI]
  21. 21 The association between emotional and behavioral problems and gastrointestinal symptoms among children with high-functioning autism [DOI]
  22. 22 Oral Human Immunoglobulin for Children with Autism and Gastrointestinal Dysfunction: A Prospective, Open-Label Study [DOI]
  23. 23 Molecular Studies of Fecal Anaerobic Commensal Bacteria in Acute Diarrhea in Children [DOI]
  24. 24 Characteristics of the gastrointestinal microbiome in children with autism spectrum disorder: a systematic review. [DOI]
  25. 25 Modern Diet and its Impact on Human Health [DOI]
  26. 26 Bifidogenic effect of whole-grain wheat during a 12-week energy-restricted dietary intervention in postmenopausal women [DOI]

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