Study on the Plasmid and Genomic DNA Stability of DH5α Host System
Laxmisai Dixitha Vengili, Phavethra Sago, Yaswanth Kumar Reddy Kuncha, Venkata Suneela Kolli, Lasya Sri Varugu, Sree Lakshmi Mandadapu, Suryanarayana Veeravilli, Praveen Kumar Vemuri
Journal of Pharmaceutical Research International · pp. 1325–1328 · Published 23 Dec 2021
10.9734/jpri/2021/v33i60B34751Abstract
Plasmids are extremely valuable as a source of DNA and for use in biotechnology. Our research goal was to develop plasmids with extremely high copy numbers for lab-scale plasmid preparations. Using the beverage method simplifies the process of obtaining a large quantity of plasmid DNA for various applications. Diet Coke as a beverage demonstrated that DNA recovery is highly adequate. Diet Sting with EcoRI and BamHI enzymes was used to detect restriction digestion with high sensitivity. However, for lab-scale preparative work, our findings show that plasmid yield can be significantly increased by using standard growth procedures and commonly used growth media.
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