The Effect of Cytokines on MicroRNA Expression in TW01 Nasopharyngeal Carcinoma Cells
Ting Wei Lee, Eng Lai Tan, Ching Ching Ng, Sook Yee Gan
Journal of Advances in Medicine and Medical Research · pp. 543–554 · Published 27 Feb 2013
10.9734/BJMMR/2013/2426Abstract
Aims: To determine the effect of cytokines, namely transforming growth factor-beta one (TGF-β1), and interleukin-6 (IL-6) on the expression of 88 cancer-related microRNAs (miRNAs) in TW01 nasopharyngeal carcinoma (NPC) cells with or without the presence of Epstein-Barr virus latent membrane protein 1 (LMP1). Methodology: TW01 and TW01-LMP1 cells were treated with cytokines. MicroRNAs were isolated from treated and untreated TW01/TW01-LMP1 cells and were subjected to RT-PCR array of 88 cancer-related microRNAs. The threshold cycle (Ct) data were analysed and fold-change in the level of gene expression was calculated based on ΔΔCt using two endogenous controls, SNORD 47 and SNORD 44. Data obtained from each treatment were compared with the data obtained from the respective control group (untreated TW01/ TW01-LMP1). Results: TGF-β1 down-regulated miR-143 in TW01 NPC cells. In TW01 cells that expressed the EBV LMP1 gene (TW01-LMP1), approximately 97% of the 88 miRNAs were up-regulated by TGF-β1. Among them was miR-181c a well-known repressor of NOTCH2/4 and KRAS and has important role in cell differentiation. IL-6 up-regulated approximately 65% of the miRNAs in TW01 cells but in less than four-fold. In TW0-LMP1 cells, eight miRNAs; namely, miR-15b, miR-155, miR-16, miR-215, miR-23b, miR-25, miR-9 and miR-98 were significantly up-regulated by IL-6. Among these, miR-15b, miR-155 and miR-25 had been reported to be elevated in NPC tissues. Conclusion: This study provides a preliminary perspective on the effects of cytokines on the expression of miRNAs in TW01 NPC cells.
References (48)
- 1 MicroRNA signatures in human cancers [DOI]
- 2 Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? [DOI]
- 3 Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers [DOI]
- 4 TGFβ in Cancer [DOI]
- 5 Activation of interleukin-6 gene expression through the NF-kappa B transcription factor. [DOI]
- 6 A Genetic Screen Implicates miRNA-372 and miRNA-373 As Oncogenes in Testicular Germ Cell Tumors [DOI]
- 7 miR-221 and miR-222 Expression Affects the Proliferation Potential of Human Prostate Carcinoma Cell Lines by Targeting p27Kip1 [DOI]
- 8 Aberrant Expression of Oncogenic and Tumor-Suppressive MicroRNAs in Cervical Cancer Is Required for Cancer Cell Growth [DOI]
- 9 Loss of miR-122 expression in liver cancer correlates with suppression of the hepatic phenotype and gain of metastatic properties [DOI]
- 10 MicroRNA-155 Is Regulated by the Transforming Growth Factor β/Smad Pathway and Contributes to Epithelial Cell Plasticity by Targeting RhoA [DOI]
- 11 MicroRNA-155 as a proinflammatory regulator in clinical and experimental arthritis [DOI]
- 12 The microRNA miR-181 targets the homeobox protein Hox-A11 during mammalian myoblast differentiation [DOI]
- 13 TGF-β activates Akt kinase through a microRNA-dependent amplifying circuit targeting PTEN [DOI]
- 14 Identification of microRNA‐181 by genome‐wide screening as a critical player in EpCAM–positive hepatic cancer stem cells† [DOI]
- 15 miR-146a Suppresses Invasion of Pancreatic Cancer Cells [DOI]
- 16 Emerging Role of miR-106b-25/miR-17-92 Clusters in the Control of Transforming Growth Factor β Signaling [DOI]
- 17 Downregulation of microRNAs‐143 and ‐145 in B‐cell malignancies [DOI]
- 18 MicroRNA deregulation and pathway alterations in nasopharyngeal carcinoma [DOI]
- 19 Exosomes released by EBV-infected nasopharyngeal carcinoma cells convey the viral Latent Membrane Protein 1 and the immunomodulatory protein galectin 9 [DOI]
- 20 Induction of cyclooxygenase-2 by Epstein–Barr virus latent membrane protein 1 is involved in vascular endothelial growth factor production in nasopharyngeal carcinoma cells [DOI]
- 21 Epstein–Barr virus latent membrane protein 1 trans-activates miR-155 transcription through the NF-κB pathway [DOI]
- 22 Identification of Novel Epstein-Barr Virus MicroRNA Genes from Nasopharyngeal Carcinomas [DOI]
- 23 miR-93, miR-98, and miR-197 Regulate Expression of Tumor Suppressor Gene FUS1 [DOI]
- 24 The significance of LMP1 expression in nasopharyngeal carcinoma [DOI]
- 25 miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice [DOI]
- 26 Combination of hsa-miR-375 and hsa-miR-142-5p as a predictor for recurrence risk in gastric cancer patients following surgical resection [DOI]
- 27 Enhancer of Zeste homolog 2 (EZH2) is overexpressed in recurrent nasopharyngeal carcinoma and is regulated by miR-26a, miR-101, and miR-98 [DOI]
- 28 microRNA-141 is involved in a nasopharyngeal carcinoma-related genes network [DOI]
- 29 Involvement of epigenetically silenced microRNA-181c in gastric carcinogenesis [DOI]
- 30 Transforming Growth Factor-β and microRNA:mRNA Regulatory Networks in Epithelial Plasticity [DOI]
- 31 miR-372 Regulates Cell Cycle and Apoptosis of AGS Human Gastric Cancer Cell Line through Direct Regulation of LATS2 [DOI]
- 32 Epstein-Barr Virus latent membrane protein 1 induces Snail and epithelial–mesenchymal transition in metastatic nasopharyngeal carcinoma [DOI]
- 33 Down-regulation of MiR-206 promotes proliferation and invasion of laryngeal cancer by regulating VEGF expression.
- 34 Upregulation of MiR-155 in Nasopharyngeal Carcinoma is Partly Driven by LMP1 and LMP2A and Downregulates a Negative Prognostic Marker JMJD1A [DOI]
- 35 Down-regulation of miR-23b may contribute to activation of the TGF-β1/Smad3 signalling pathway during the termination stage of liver regeneration [DOI]
- 36 The Epstein-Barr virus and its association with human cancers. [DOI]
- 37 The polycomb group protein enhancer of zeste homolog 2 (EZH2) is an oncogene that influences myeloma cell growth and the mutant ras phenotype [DOI]
- 38 MicroRNA-372 Is Down-regulated and Targets Cyclin-dependent Kinase 2 (CDK2) and Cyclin A1 in Human Cervical Cancer, Which May Contribute to Tumorigenesis [DOI]
- 39 miR-335 Directly Targets Rb1 (pRb/p105) in a Proximal Connection to p53-Dependent Stress Response [DOI]
- 40 Expression of miR-143 reduces growth and migration of human bladder carcinoma cells by targeting cyclooxygenase-2.
- 41 Interleukins, laminin and epstein - barr virus latent membrane protein 1 (EBV LMP1) Promote metastatic phenotype in nasopharyngeal carcinoma [DOI]
- 42 Oncostatin M and Interleukin 6 inhibit cell cycle progression by prevention of p27kip1 degradation in HepG2 cells [DOI]
- 43 Epstein–Barr virus-encoded LMP1 induces a hyperproliferative and inflammatory gene expression programme in cultured keratinocytes [DOI]
- 44 Notch inhibition suppresses nasopharyngeal carcinoma by depleting cancer stem-like side population cells [DOI]
- 45 Endogenous TGF‐β contributes to the induction of the EBV lytic cycle in two burkitt lymphoma cell lines [DOI]
- 46 Elevated Serum Transforming Growth Factor β1 Levels in Epstein-Barr Virus-Associated Diseases and Their Correlation with Virus-Specific Immunoglobulin A (IgA) and IgM [DOI]
- 47 Response of Nasopharyngeal Carcinoma Cells to Epstein-Barr Virus Infection In Vitro [DOI]
- 48 Effects of eb virus encoded LMP1 on differentiation of nasopharyngeal carcinoma cells [DOI]
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