The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity
Zamree Md Shah, Mohd Kamal Nik Hasan, Khairul Kamilah Abdul Kadir, Mohd Shahidan Mohd Arshad, Zulkhairi Amom
Asian Food Science Journal · pp. 106–120 · Published 30 Mar 2021
10.9734/afsj/2021/v20i430291Abstract
The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.
References (22)
- 1 The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay [DOI]
- 2 Response surface methodology (RSM) as a tool for optimization in analytical chemistry [DOI]
- 3 Extraction and analysis of phenolics in food [DOI]
- 4 Optimization of extraction of phenolic compounds from wheat using response surface methodology [DOI]
- 5 Optimization of phenolics and dietary fibre extraction from date seeds [DOI]
- 6 Microwave-assisted extraction of tea phenols: A phenomenological study [DOI]
- 7 Antioxidant Activity of Extracts from Du-zhong (Eucommia ulmoides) toward Various Lipid Peroxidation Models in Vitro [DOI]
- 8 Antimalarial activity of extracts of Malaysian medicinal plants [DOI]
- 9 Tinospora crispa (L.) Hook. f. & Thomson: A Review of Its Ethnobotanical, Phytochemical, and Pharmacological Aspects [DOI]
- 10 Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology [DOI]
- 11 Optimization and validation of a methodology based on solvent extraction and liquid chromatography for the simultaneous determination of several polyphenolic families in fruit juices [DOI]
- 12 Herbs for medicinal baths among the traditional Yao communities of China [DOI]
- 13 Antidiabetic effects of Tinospora crispa in rats [DOI]
- 14 Study on cardiac contractility of cycloeucalenol and cycloeucalenone isolated from Tinospora crispa [DOI]
- 15 Antioxidant properties: effects of solid-to-solvent ratio on antioxidant compounds and capacities of pegaga (Centella asiatica)
- 16 Characterization of material parameters of composite plate specimens using optimization and experimental vibrational data [DOI]
- 17 Long-term reduction of C-reactive protein concentration by regular LDL apheresis [DOI]
- 18 Phenolic content and antioxidant capacity of supercritical carbon dioxide-treated and air-classified oat bran concentrate microwave-irradiated in water or ethanol at varying temperatures [DOI]
- 19 Biological Properties of Tinospora crispa (Akar Patawali) and Its Antiproliferative Activities on Selected Human Cancer Cell Lines.
- 20 A Review of the Secondary Metabolites and Biological Activities of Tinospora crispa (Menispermaceae) [DOI]
- 21 Inhibitory Properties of Tinospora crispa Extracts on TNF-a Induced Inflammation on Human Umbilical Vein Endothelial Cells (HUVECS) [DOI]
- 22 1,1-Diphenyl-2-picrylhydrazyl Radical Scavenging Compounds of Fraxini Cortex
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