The Protective Role of Extract of Allium cepa Linn. (Liliaceae) (Red Onion) Bulb in Artesunate-induced Testicular Damage in Male Wistar Rats
Nwaehujor Chinaka O, Igile Godwin O, Nwinyi Florence C, Ode Julius O.
Journal of Pharmaceutical Research International · pp. 1715–1724 · Published 10 Jul 2014
10.9734/BJPR/2014/10675Abstract
Aim: Allium cepa is consumed fresh or cooked in Nigeria and used as herb in ethno-medicinal practice against many ailments. The aqueous extract is used in the management of prostrate inflammation, alleviation of low sperm count and testicular damage related disorders in ethno medicine and folkloric medicine in the South-east regions of Nigeria. This study investigates the protective role of extract of Allium cepa bulb on spermatogenesis and testicular oxidative stress in male rats using Artesunate as testicular damage and oxidative stress inducing agent. Study Design and Methodology: The design consisted of 5 groups of 10 male Wistar rats (140-180g) each. Groups 1, 2, 3 and 5 were given continuous oral challenge with 4.4mg/kgb.w Artesunate orally for two weeks to induce testicular damage. Animals were treated with 50, 150 and 300mg/kgb.w extract for 7 weeks. Groups 4 and 5 were not treated and served as normal and negative controls respectively. Animals were humanely euthanized, testes collected, homogenized and used for sperm count, motility and oxidative stress evaluation. Results: Artesunate mediated testicular damage caused a significant (p<0.01) reduction in sperm count (17.5±2.4x106ml/l) compared to normal control (140X106ml/l). Extract treatment of groups 1, 2 and 3 caused a dose-dependent reversal of sperm count, motility and morphology after 7 weeks. Treatment with the highest dose (300 mg/kg) of the extract reversed testicular oxidative induced lesions as shown by levels of enzymatic antioxidants; CAT (10.112±1.73µg/mg), SOD (39.41±2.35µg/mg) and GPx (0.55±0.17nmol/mg) and non-enzymatic antioxidants; MDA (0.98±0.05nmol/mg) and GSH (2.17±0.05mg) relative to normal control, respectively. Conclusion: These results demonstrated the potential beneficial effects of Allium cepa in the management of prostrate inflammation and testicular lesions.
References (21)
- 1 Tissue sulfhydryl groups [DOI]
- 2 Methods of Enzymatic Analysis [DOI]
- 3 [30] Microsomal lipid peroxidation [DOI]
- 4 Selenium: Biochemical Role as a Component of Glutathione Peroxidase [DOI]
- 5 The estimation of red cell superoxide dismutase activity.
- 6 The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats [DOI]
- 7 Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa) [DOI]
- 8 Antioxidant nutrients and adriamycin toxicity [DOI]
- 9 Review of the clinical pharmacokinetics of artesunate and its active metabolite dihydroartemisinin following intravenous, intramuscular, oral or rectal administration [DOI]
- 10 Hypoglycaemic and antioxidant effects of onion,Allium cepa: dietary onion addition, antioxidant activity and hypoglycaemic effects on diabetic rats [DOI]
- 11 Potentiation of Doxorubicin Cardiotoxicity by Iron Loading in a Rodent Model [DOI]
- 12 Multiple dose pharmacokinetics of artemether in Chinese patients with uncomplicated falciparum malaria [DOI]
- 13 An onion variety has natural antithrombotic effect as assessed by thrombosis/thrombolysis models in rodents [DOI]
- 14 Antioxidative and Cardioprotective Effects of Phyllanthus urinaria L. on Doxorubicin-Induced Cardiotoxicity [DOI]
- 15 Amifostine protects against early but not late toxic effects of doxorubicin in infant rats.
- 16 Lipoic acid ameliorates adriamycin-induced testicular mitochondriopathy [DOI]
- 17 The effects of mono-2-ethylhexyl phathalate, adriamycin and N-ethyl-N-nitrosourea on stage-specific apoptosis and DNA synthesis in the mouse spermatogenesis [DOI]
- 18 Acute Hepatotocixity Following Administration Of Artesunate In Guinea Pigs [DOI]
- 19 Influence of urethane and ketamine on rat hepatic cytochrome P450 in vivo [DOI]
- 20 Antidiabetic activity of Egyptian onion "Allium cepa" extract.
- 21 The effects of quinine and artesunate treatment on plasma tumor necrosis factor levels in malaria-infected patients.
Cited by 8
Solomon Tsekohol Agu, Akintola Ahmed Alabi, Deborah Oluwaseun Meshioye · The Journal of Basic and Applied Zoology · 2023
Oyewusi J. Adeoye, Oridupa A. Olayinka, Saba A. Bernard · Journal of Complementary and Integrative Medicine · 2018
Mariam M. Elattar, Hala M. Hammoda, Doaa A. Ghareeb · BMC Complementary Medicine and Therapies · 2024
Showing 3 of 8 known citations — external sources report more than can currently be individually listed.
Related research
- Salivary Lipid Peroxidation and Antioxidant Status in Nigerian Cigarette Smokers with or Without Periodontitis — shares topic coverage
- Profile of Biochemical and Oxidative Stress Markers in Male Athletes Following Strenuous Exercise Session — shares topic coverage
- Atherogenicity, Oxidative Stress, Heavy Metals and Bioelements Status in Hypertensive Nigerians in an Urban Population — shares topic coverage
- Oxidative Stress Biomarkers (Malondialdehyde, Superoxide Dismutase, and Catalase) as Predictors of Cardiovascular Disease Risk: A Review — shares topic coverage
- Ebselen a Seleno-organic Molecule Inhibits Alteration in the Biological Responses in Hypoxic Human Alveolar Lung Epithelial Cells — shares topic coverage
Article metrics
Real usage data collected on this platform.
4
Page views
0
PDF downloads
0
Outbound clicks
8
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.