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Research Article Open access CC BY 3.0

Transgenic Investigation of Canola (Brassica napus L.)

Sultan Fidan, Ercan Arican

Annual Research & Review in Biology · pp. 4293–4304 · Published 8 Aug 2014

10.9734/ARRB/2014/11684

Abstract

Investigating transgenic characteristics of Californium, Jura, Elvis and Orkan varieties of canola has been carried out in this study. These plants are widely cultivated in Turkey. The Canola varieties were grown in an established tissue culture and the total DNA isolated. The 35S and pNOS promoter region of the transgene were scanned using classic and multiplex PCR techniques. The transfer of transgenic characteristic to the NAD gene region confirms the accuracy of the PCR technique. This study, has determined that Californium, Jura, Elvis and Orkan varieties of canola consist of transgenic characteristics and all the four varieties have multiple insertion region in terms of CaMV 35S and Pnos promoters.

Canola transgenic geneticly modified organisms pNOs promoter 35S promoter multiplex PCR

References (26)

  1. 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
  2. 2 Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress [DOI]
  3. 3 PLANT TRANSFORMATION: Problems and Strategies for Practical Application [DOI]
  4. 4 Unintended effects and their detection in genetically modified crops [DOI]
  5. 5 The impact of genetic modification of human foods in the 21st century [DOI]
  6. 6 Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences [DOI]
  7. 7 Expression and inheritance of multiple transgenes in rice plants [DOI]
  8. 8 Housekeeping Genes for Phylogenetic Analysis of Eutherian Relationships [DOI]
  9. 9 High-efficiency transformation of Gossypium hirsutum embryogenic calli mediated by Agrobacterium tumefaciens and regeneration of insect-resistant plants [DOI]
  10. 10 Monitoring the occurrence of genetically modified maize at a grain receiving port and along transportation routes in the Republic of Korea [DOI]
  11. 11 Real-time PCR method for detection of the transgenic rice event TT51-1 [DOI]
  12. 12 Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens [DOI]
  13. 13 A general multiplex-PCR assay for the general detection of genetically modified soya and maize [DOI]
  14. 14 Development of melting temperature-based SYBR Green I polymerase chain reaction methods for multiplex genetically modified organism detection [DOI]
  15. 15 Transformation of sunflower (Helianthus annuus L.) following wounding with glass beads [DOI]
  16. 16 Multiplex qualitative PCR assay for identification of genetically modified canola events and real-time event-specific PCR assay for quantification of the GT73 canola event [DOI]
  17. 17 Multiple gene co-integration in Arabidopsis thaliana predominantly occurs in the same genetic locus after simultaneous in planta transformation with distinct Agrobacterium tumefaciens strains [DOI]
  18. 18 Event-specific qualitative and quantitative PCR detection of genetically modified rapeseed Topas 19/2 [DOI]
  19. 19 Sampling, detection, identification and quantification of genetically modified organisms (GMOs) [DOI]
  20. 20 Toward increasing erucic acid content in oilseed rape (Brassica napus L.) through the combination with genes for high oleic acid [DOI]
  21. 21 Detection of GM soybean in food products by simultaneous employment of three pairs of PCR primers [DOI]
  22. 22 Quantitative competitive PCR for the detection of genetically modified organisms in food [DOI]
  23. 23 Detection of Genetically Modified Crops by Combination of Multiplex PCR and Low-density DNA Microarray [DOI]
  24. 24 Development of a polymerase chain reaction assay for detection of three canola transgenes [DOI]
  25. 25 Screening of transgenic plants by multiplex PCR [DOI]
  26. 26 Fluorophore double stranded probe-multiplex quantitative PCR method for detecting transgenic component of promoter derived from Cauliflower Mosaic Virus and nos terminator derived from Agrobacterium tumefaciens simultaneously [DOI]

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