Skip to content
Research Article Open access CC BY 3.0

Xanthine Oxidase Inhibition and Antioxidant Effects of Peaganum harmala Seed Extracts

A. Baghiani, M. Djarmouni, S. Boumerfeg, H. Trabsa, N. Charef, S. Khennouf, L. Arrar

European Journal of Medicinal Plants · pp. 42–56 · Published 7 Feb 2012

10.9734/EJMP/2012/898

Abstract

In the present study, seeds of Peaganum harmala were extracted with solvent of varying polarity allowed their separation into four subfractions. Total polyphenols and flavonoids contents of Peaganum harmala seeds extracts (PHSE) were determined as gallic acid equivalent and Quercetin equivalent and were in the following order: chloroform extract (CHE)>ethyl acetate extract (EAE)>crude extract (CE). The superoxide radical scavenging properties of PHSE were determined by either production of uric acid and reduction of Cytochrom c (Cyt c). The inhibition ratios of the Xanthine Oxidase (XO) by CHE and EAE were effective followed by CE. Results for the Cyt c assay were in the same order. The antioxidant potential was confirmed with the non enzymatic methods. The highest DPPH radical scavenging activity was observed with CE followed by EAE and CHE. The β-carotene/linoleic acid bleaching assay revealed that PHSE have a very important antioxidant activity. Results showed that EAE has the highest antioxidant activity (94.21%), followed by CE and CHE respectively. Ferrous ion chelating capacity assay showed that CE, EAE and CE are very active with IC50 of 0.088, 2.256 and 5.286 mg/ml, respectively. Peaganum harmala extracts have strong free radicals scavenging and antioxidant activities and may prove to be of potential health benefit. In addition, the ability of PHSE to inhibit XO can be of great use in some diseases where XO is incriminated.

Xanthine Oxidase Peaganum harmala antioxidant free radicals scavengers polyphenols

References (33)

  1. 1 Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 [DOI]
  2. 2 Use of a free radical method to evaluate antioxidant activity [DOI]
  3. 3 Antioxidant Determinations by the Use of a Stable Free Radical [DOI]
  4. 4 Flavonoids: antioxidants or signalling molecules? [DOI]
  5. 5 Flavonoids are scavengers of superoxide anions [DOI]
  6. 6 Techniques of flavonoid identification.
  7. 7 Structure−Activity Relationship and Classification of Flavonoids as Inhibitors of Xanthine Oxidase and Superoxide Scavengers [DOI]
  8. 8 Antioxidant activities of aqueous extracts of selected plants [DOI]
  9. 9 Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain [DOI]
  10. 10 Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties [DOI]
  11. 11 Antioxidants and Human Disease: A General Introduction [DOI]
  12. 12 Antioxidant activities of buckwheat extracts [DOI]
  13. 13 Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania [DOI]
  14. 14 Evaluation of antioxidant activities and total phenolic contents of typical malting barley varieties [DOI]
  15. 15 Stabilization of the Prussian blue color in the determination of polyphenols [DOI]
  16. 16 Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations.
  17. 17 Preparation and antioxidant properties of water extract of propolis [DOI]
  18. 18 Radical scavenging activity and antioxidant capacity of bay leaf extracts [DOI]
  19. 19 Xanthine oxidase inhibitory activity of some Indian medical plants [DOI]
  20. 20 Dandelion (Taraxacum officinale) flower extract suppresses both reactive oxygen species and nitric oxide and prevents lipid oxidation in vitro [DOI]
  21. 21 Antioxidant Activity of Hypericum androsaemum Infusion: Scavenging Activity against Superoxide Radical, Hydroxyl Radical and Hypochlorous Acid. [DOI]
  22. 22 Antinociceptive effects of Peganum harmala L. alkaloid extract on mouse formalin test.
  23. 23 Ascorbic acid and beta-carotene as modulators of oxidative damage [DOI]
  24. 24 Xanthine oxidase inhibitory activity of Lychnophora species from Brazil (“Arnica”) [DOI]
  25. 25 Antioxidant and Antimicrobial Properties of the LichensCladonia foliacea.,Dermatocarpon miniatum.,Everinia divaricata.,Evernia prunastri., andNeofuscella pulla. [DOI]
  26. 26 Peganum harmala L: a poisonous plant of North Africa.
  27. 27 The influence of electronic, steric and hydrophobic properties of flavonoid compounds in the inhibition of the xanthine oxidase [DOI]
  28. 28 Antioxidant properties and xanthine oxidase inhibitory effects of Tamus communis L. root extracts [DOI]
  29. 29 Purification and Partial Characterisation of Camel Milk Xanthine Oxidoreductase [DOI]
  30. 30 Antioxidant and Radical Scavenging Properties of Carthamus caeruleus L Extracts Grow Wild in Algeria Flora [DOI]
  31. 31 253. Cellular constituents. The chemistry of xanthine oxidase. Part III. Estimations of the co-factors and the catalytic activities of enzyme fractions from cow's milk [DOI]
  32. 32 Effets de l’alimentation en Peganum harmala L. (Zygophyllaceae) sur le tube digestif du criquet pèlerin Schistocerca gregaria Forsk. (Orthoptera, Acrididae).
  33. 33 Free radicals in biology and medicine [DOI]

Cited by 8

Wound-healing activity of Algerian Lavandula stoechas and Mentha pulegium extracts: from traditional use to scientific validation

Faiza Baali, Sabah Boumerfeg, Amel Boudjelal · Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology · 2021

Acute, sub-acute toxicity and antioxidant activities (in vitro and in vivo) of Reichardia picroide crude extract

Sana Aouachria, Sabah Boumerfeg, Abderrahim Benslama · Journal of Ethnopharmacology · 2017

Showing 2 of 8 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

34

Page views

0

PDF downloads

6

Outbound clicks

8

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.